The cross-section for prompt antiproton production in collisions of protons with an energy of $6.5$ TeV incident on helium nuclei at rest is measured with the LHCb experiment from a data set corresponding to an integrated luminosity of $0.5\,nb^{-1}$. The target is provided by injecting helium gas into the LHC beam line at the LHCb interaction point. The reported results, covering antiproton momenta between $12$ and $110\,\mathrm{GeV/}c$, represent the first direct determination of the antiproton production cross-section in ${\rm p He}$ collisions, and impact the interpretation of recent results on antiproton cosmic rays from space-borne experiments.
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